The Colorado potato beetle (CPB) is a major agriculture pest of potato, tomato and eggplant leading to serious crop production loss worldwide. Currently, use of pesticides is the main management practice to control CPB. However, CPB has a robust ability to develop resistance to all major classes of pesticides used for its control. One of the major mechanisms contributing to the development of pesticide resistance in CPB, is upregulated expression of detoxification enzymes including Cytochrome P450s, Carboxylesterases (CCEs), Glutathione S-transferases (GSTs), UDP-glycosyl transferases (UGTs) and ATP binding cassette transporters (ABC transporters). GSTs constitute a large family of multifunctional enzymes that are involved in Phase II metabolic detoxification of pesticides. GSTs catalyze the conjugation reaction of lipophilic compounds with the thiol group of reduced glutathione, resulting in more water-soluble and less toxic products that can be excreted out of organisms. Arthropod GSTs can confer adaptation to pesticides through metabolic detoxification, or by sequestration of pesticides, or by metabolism of oxidative stress products resulting from pesticide exposure. To date, the roles of GSTs in pesticide adaptation in CPB have not been well studied. Here, a novel GST termed LdGST8 was identified from the CPB transcriptome and its structure and functions have been characterized.