(HPB P3) Analysis of Cholyglycine Acid as a Biomarker for the Early Diagnosis of Liver Disease
Saturday, February 15, 2020
12:15 PM – 12:20 PM
Trace concentrations of cholyglycine acid (CG) are useful to reflect the damage degree in liver cells, and useful in the diagnosis and prognosis of liver diseases. Therefore, developing an effective strategy to detect the concentration of CG is of great importance. A homogeneous fluorescence polarization immunoassay (FPIA) was developed for determination of CG concentrations. The effects of tracer concentration, antibody dilution and incubation time on FPIA performance were detected. The results were compared with those obtained through enzyme multiplied immunoassay technique (EMIT). The FPIA showed a detection limit of 50.9 ng/mL for CG and the cross-reactivity of antibodies with nine structurally and functionally related analogs were negligible. Concentrations of CG with chronic hepatitis B virus, liver cirrhosis and decompensated cirrhosis were higher than those in controls, respectively, and hence were significantly different from controls (P<0.001). The FPIA developed in this study is a rapid, convenient, and simple method, suitable to be used as a screening tool for homogeneous detection of CG in serum. The detection results of FPIA demonstrate that concentrations of CG were much higher in liver patients than in controls. Therefore, CG may be a good biomarker for the diagnosis of liver diseases.