Efficient execution of large drug screens requires innovation in instrumentation and data analysis to more rapidly identify potential drug candidates. In this presentation, we will demonstrate how the Intellicyt® iQue3 advanced flow cytometry platform uses walkaway automation tools such as plate loading, calibration, cleaning and intelligent reagent level sensing to simplify your drug screening studies. Using a structure activity relationship (SAR) compound library screen on PBMCs, we measured multiple parameters including cell viability, immunophenotyping and cytokine secretion in a miniaturized assay format on the iQue3. We overcame the data analysis bottleneck associated with screening experiments using our integrated ForeCyt Software package. Compounds that promote cytokine and T cell activation were identified by rapid, multiparametric data analysis and novel data visualization, including multiplate data analysis using our Panorama feature. In summary, the Intellicyt iQue platform offers automated set up, acquisition and data analysis to collect and interpret 18,000 data points quickly and reliably, resulting in faster time to actionable results.