Demonstrating translational relevance of organoid and multicellular tissues for preclinical models of human disease
Intestinal organoid technologies have revolutionized culture models to study physiology, disease, and injury in vitro. While primary stem cell-driven organoid cultures offer many improvements over conventional cancer cell line models, individual organoids are highly heterogeneous in lineage ratios, morphologies, growth properties, and other physiological parameters. Additionally, the enclosed lumen prohibits easy access to the apical cell surface to study nutrient absorption, the microbiome, and drug interactions with the epithelium. We have developed platforms that address these challenges. Specifically, our group focuses on engineering high-throughput systems to study single-cell stem cell biology, stem cell niche co-cultures, organoid dynamics, luminal physiology, and the microbiome. These platforms can be applied to organoids across any tissue type, are scalable, portable, and represent a high-resolution and statistically robust solution for preclinical models of human disease.