There has been much focus in the industry for many years on the quality of compounds within screening collections and the impact of storage conditions on overall compound purity and as a result, screening libraries have improved over the years. There has also been some effort placed into understanding the susceptibility of assay formats to particular chemical classes but little has been done to evaluate the impact of the target proteins themselves.
We will share our data where we retrospectively reviewed the purity of hit compounds identified from high throughput screening campaigns to look for trends in the overall compound purity pass rates. This study has revealed that the nature of the target class screened as well as the assay conditions lead to a marked difference in the overall number of compounds that pass set purity thresholds. These data can then be used to determine appropriate quality control processes, suitable assay conditions for a particular target class and guide decisions on library composition and the selection of new compounds to be added to a screening collection.