Automation and High-Throughput Technologies
Screening & Profiling at higher throughput using physiologically relevant cell models
Alzheimer’s disease (AD) is a complex disorder with increasing prevalence and socio-economic burden. However, majority of strategies aimed at identifying therapies for AD have been focused on targeting Abeta or TAU, which make up the plaques and tangles respectively commonly found in people with AD. Continued failure of the drug discovery process and the accompanying trials against these targets has necessitated more and better options for therapeutic intervention.
Using a multi-parametric high content phenotypic readouts with neurons derived from human differentiated iPSCs with familial AD mutations, we will perform CRISPR based rescue screen for the various phenotypes associated with the mutations, such as endolysosomal transport, synaptic dysfunction, neuronal toxicity. The multiple-phenotypic rescue approach will enable identification novel key pathways and/or targets which could serve has drug candidates for the treatment of AD