Single cell isolation is essential in stem cell biology, cancer research and biotechnology among others. For example, to ensure quality, safety and efficacy of the biotherapeutic product, companies shall demonstrate that each new recombinant cell line has been cloned from a single progenitor cell (WHO, 2014). Because available methods for cell cloning do not provide fully traceable cells yet, companies may waste up to 50 weeks in clonal validation. To solve this issue, we have developed an automated impedance-based pipetting robot for single cell dispensing, allowing for traceable cloning of single cells. This technology permits the efficient and gentle isolation of industrial cell lines as well as rare and fragile stem cells and cancer cells, at a single cell resolution, so that cells can be individually expanded in culture, transplanted downstream or analyzed by omics assays. We will present the technology and illustrate its key features through various case studies.