Many labs turn to automation to increase throughput, lower costs, and reduce operator-related errors. Transferring a manual, bench-top assay to an automated platform, however, is often more complicated than expected. By understanding the key steps in complicated workflows such as next generation sequencing (NGS) and taking steps to minimize variability upfront, successful assay automation can be facilitated which can save time. Examples of key steps in the process include DNA normalization, which requires a unique volume of diluent and DNA (both typically <10 µL) for each sample and addition of a master mix solution, which is typically viscous. We will highlight our approach to improving these critical steps as well as discuss our methodology for ensuring consistent pipetting for manual operations. A genomics and automation expert will also discuss how to set up an automated NGS workflow using the Tecan DreamPrep, consisting of the Fluent® Automation Workstation, Infinite® F Nano+ plate reader and Tecan Genomics’ library preparation kits to provide sequencing-ready libraries. Details on how to optimize this workflow will be presented.