Organ-on-a-Chip technology is a new paradigm in drug testing. The aim of this technology is to raise the physiological relevance of traditional cell culture by combining it with microfluidic techniques. Organs-on-a-Chip are 3D tissues that capture the complexity of in vivo tissues including 3D morphology, extracellular matrix embedment, multiple cell types, vascular structure and perfusion flow. Incorporating these technologies in a microplate format enables increased throughput in high content imaging systems.
In this presentation, an expert from MIMETAS will introduce the culture and assay of models resembling complex 3D tissues, such as liver, kidney, gut and brain tissue. The tissues are grown in the OrganoPlate®, an in vitro cell culture microplate platform that allows culturing of over 40 tissues in parallel. The tissues are embedded in an extracellular matrix gel and comprise both stromal tissue, blood vessel structures as well as epithelial barriers.
These 3D tissue cultures can be interrogated in a high-throughput manner using powerful cellular imaging systems. We will show how Molecular Devices ImageXpress® Micro Confocal High-Content Imaging System equipped with water immersion objectives and ImageXpress Pico Automated Cell Imager enable automated image acquisition, visualization and multi-parametric analysis of complex 3D tissue models built on the OrganoPlate platform.
We will show examples of Organ-on-a-Chip tissues for use in disease modeling and drug safety testing and will present the new OrganoPlate Graft, which allows vascularization of spheroids, organoids and PDX explants, offering a powerful solution for the study of angiogenesis, an important focus for cancer therapeutics.