Rice (Oryza sativa L.) is major crops providing calories for people worldwide. The US is among the top global exporters and Arkansas produces ~50% of all domestic rice. The rice production is highly affected by increasing global air temperatures. High night air temperature stress affects both rice yield and grain quality. The responses of rice to high night air temperature stress have been analyzed in limited number of genotypes mostly under greenhouse conditions. One of the challenges for implementing these studies under field conditions is the lack of high throughput phenotyping infrastructure. The physiological and metabolic responses of rice to high night air temperature stress under field conditions are not yet fully elucidated, thus, further studies are needed. In this work, new infrastructure consisting of six high tunnel greenhouses fitted with sensors and heating systems were successfully established in a state-of-the-art field experimental station in Harrisburg, AR; 310 rice accessions from the Rice Diversity Panel 1 (RDP1) and 10 hybrids from RiceTec Inc. were grown in each greenhouse arranged in a randomized block design. During flowering, three of the greenhouses were successfully and uniformly heated at night for two weeks. Air temperature in the three heated greenhouses was 3-4oC higher than ambient temperature controls as recorded with Raspberry Pi-powered systems. This temperature differential was confirmed with HOBO temperature data loggers. These greenhouses were able to withstand harsh field conditions including constant flooding, strong rain, and 40-50 mph winds. Yield analysis, seed quality assessment, and other biochemical assays are still ongoing to fully assess the effects of high night air temperature stress in the RDP1 and hybrids of interest. These studies will lead to identification of novel markers that can be used by rice breeders and molecular biologists to develop rice varieties more resilient to heat stress.