Increased understanding of plant systems improves our ability to grow more stress-resilient crop species and meet increasing food demand. High-throughput phenotyping accelerates plant research by enabling experiments with more repetitions and a greater genetic diversity to identify unique physiological traits. The new high-throughput phenotyping service at Washington State University is built into a light/temperature/humidity/CO2-controlled growth chamber that facilitates studies of the effect of environmental stresses and genetic adaptions. The platform carries a suite of cameras for visible RGB, photon counting, pulsed chlorophyll fluorescence, and shortwave-infrared imaging systems, as well as a 3D laser scanner. It includes a 42-tray hotel for growing plants with automatic watering. After a year of operations, we’ve completed a dozen experiments with a variety of plant species including arabidopsis, (micro)tomatoes, and chickpeas that each presented unique challenges. We present the lessons learned from developing protocols to capture high quality images that will help advance our understanding of plant systems. We are accepting proposals for pilot studies as we transition to a paid-use resource for the plant community at WSU and abroad.