Research Biologist Agriculture and Agri-Food Canada
Disclosure: Disclosure information not submitted.
In attempts to identify an optimal root system architecture for Brassica, 50 B. napus founder genotypes of a nested association mapping (NAM) population comprising 2500 recombinant inbred lines (RILs) were examined to capture the natural variation in RSA. The NAM population was derived from crosses between each of the 50 founders and a common parent, and later genotyped using a 60K Brassica Infinium SNP array at Agriculture and Agri-Food Canada's (AAFC) Saskatoon Research and Development Centre (SRDC). Plant Root Imaging and Data Acquisition (PRIDA®) software enabled 2D image capture of hydroponically grown 14-18 day old seedling roots at the Global Institute for Food Security (GIFS) in Saskatoon. Root 2D images were processed in order to quantify root traits (for e.g. total root length, tap and lateral root length, lateral root number and density, root hair density etc.). The above ground phenotype of the same Brassica NAM founder lines under optimal and water limiting conditions were captured in a LemnaTec 3D High Throughput Scanalyzer. A subset of progeny lines derived from founders with extreme RSA and/or response to water limiting conditions will be 2D imaged to generate datasets to facilitate QTL, GWAS analyses, and identifcaition of trait-specific molecular markers. Further, given the low nitrogen use efficiency (NUE) of Brassica spp., select progeny with extreme RSA will be assessed for NUE in the field. A similar analysis of a B. carinata NAM population (50 founders and 2500 progeny) will help detect potetial novel variation with respect to QTL/genes on the C genome shared with B. napus, along with improving the genetic mapping resolution.