Sorghum stalk lodging can significantly reduce harvest yields and economic loss for farmers. Therefore, understanding the properties of the stalks (mechanical properties, cell wall chemistry, cell morphology, etc) and how it influences lodging would be useful in developing new breading lines that are resistant to lodging. In this study the cell wall chemistry of two sorghum varieties, genetically modified brown midrib mutant (bmr) with reduced lignin content and unmodified wild type (WT), were investigated. In relation to understanding and correlating crop lodging to cell wall chemistry the sorghum stalks were evaluated at the nodes and internodes for their axial variation of chemical composition by various techniques ( FTIR, XRD, lignin and carbohydrate contents, Py-GCMS, and 2D 1H-13C NMR ). The carbohydrate analysis showed that nodes and internodes of the two varieties in the axial direction have different carbohydrates,and lignin contents. 2D NMR showed that the lignin is rich in b-O-4 linkage, however, phenylcoumaran, dibenzodioxocin, and resinol linkages were not detected. The aromatic regions for bmr highlights that the syringyl units were not visible, but for the WT, syringyl units were able to detected in the spectrum in all nodes and internodes. This work shows that differences in chemical composition of the stalks could influence its mechanical properties and lodging behavior.