Benzoxazinoids such as DIMBOA constitute an important class of secondary metabolites. These compounds act as both defense and signaling molecules which are found in both above and below ground plant structures. Additionally, when exuded in roots, benzoxazinoids play a number of roles including recruitment of beneficial microbes. Specifically, there has been some work showing that the seminal roots are a major source of DIMBOA and while it is known that DIMBOA is produced throughout the plant, the spatiotemporal variance and amounts exuded in the various root structures is less characterized. Using ferric chloride, a chemical indicator with specificity for DIMBOA containing compounds, we are developing a high throughput method to localize and quantify this exudate. To assess this variance in exudation throughout maize roots our experiment will utilize nine genotypes at two time points. By using nine genotypes we may be able to discern patterns in exudation between them. Plants are grown vertically on absorbent blotting paper, the bottom of which is submerged in a calcium chloride solution. Exudates are absorbed onto a nylon substrate using a simple, yet uniform blotting method. After blots are dried DIMBOA in known concentrations is pin replicated onto the substrate which will allow for the creation of a standard curve to quantify exudate absorbed onto the substrate surface. Ferric chloride is printed onto the blots which are then scanned on flatbed scanners. Finally, an automated image processing platform has been created to build standard curves and quantify root exudation signals. Ultimately, this analysis will yield insights into total DIMBOA production over time and spatial variance between genotypes.