Vice President Scientific Support Meso Scale Discovery SAN DIEGO, California
Many studies are underway to assess single nucleotide polymorphisms (SNPs) in circulating SARS-CoV-2 strains; however, these studies mostly rely on data from low-throughput and costly sequencing approaches. To mitigate these challenges, we sought to develop a more cost effective and higher throughput assay for four common SARS-CoV-2 SNPs that are implicated in viral pathogenesis and/or transmission. In this presentation, we will review how we developed a novel high throughput, plate-based assay to simultaneously detect multiple SNPS in the SARS-CoV-2 genome using a combination of oligonucleotide ligation paired with high sensitivity electrochemiluminescence detection. Results obtained from COVID-19 patients with the multiplex panel that demonstrate the performance and accuracy of the assays will be presented.
Understand why alternatives to costly, time consuming techniques such as whole genome sequencing are warranted for the detection of SARS-CoV-2 SNPs.
Understand the underlying principles of the N-PLEX multiplex platform on which this assay was developed
Learn how this technology can be implemented to rapidly detect multiple SNPs using four SARS-CoV-2 SNPs as an example.
Learn how SNPs can be used to differentiate between strains of SARS-CoV-2 implicated in viral pathogenesis and/or transmission