PhD Candidate University of Missouri Kansas City Kansas City, Missouri
We screened thousands of compounds and identified several drugs with improved anti-MRSA activities after they undergo human liver microsomal metabolism or in presence of Cefoxitin, a known antibacterial agents which has a high MIC against MRSA F-182. We used liquid handling workstations and LC-MS based analysis to screen and characterize active agents. We further extended screening some of the hits from the screening against additional MRSA strain, one VRE and one E. coli strain for spectrum of activity and frequency of resistance. This approach can be adopted for exploring other biological activities of these library of compounds with modifications. We are in the process of exploring the mechanism of action of these interesting agents using multi-omics study consisting of metabolomics, proteomics and genomics.
Upon completion, participant will be able to understand a novel approach to screen biological activity of chemical libraries in a high throughput fashion.
Upon completion, participant will be able to discuss human liver microsome metabolism in large scale and identification of active drug metabolites.
Upon completion, participant will be able to discuss ways to explore combination effects of multiple drugs (synergy or antagonoism).