BMS has established a robust and scalable next generation cell culture platform for high-productivity manufacturing of monoclonal antibodies (mAbs) by recombinant Chinese Hamster Ovary (CHO) cells. This presentation focuses on key strategies to develop the intensified fed-batch platform, including perfusion operation at N-1 seed culture step, optimization of basal and feed media and other process parameters in the production bioreactor. The process has been successfully scaled up to both pilot and manufacturing scales for multiple assets, demonstrating the scalability of the process for large scale cell culture manufacturing.
Understand process intensification with N-1 perfusion
Learn the key strategies used at BMS to establish the intensified fed-batch platform
Learn what are the current challenges with N-1 perfusion and process intensification