Track: Formulation and Delivery - Biomolecular - Drug Delivery - Other
Category: Poster Abstract
Exosomes as Nano Delivery Systems for Treatment of Non-Small Cell Lung Cancer
Purpose: The use of exosomes as nanosystems for drug delivery is an emerging field of research. Exosomes are biologically-derived nanoparticles that function by communicating between cells. The presence of various adhesion molecules on their surface enables exosomes to act as carriers for other biological molecules such as proteins, lipids, and nucleic acids to specific targets. For critical and aggressive illnesses, such as cancer, targeted therapy has become more desirable. Non-small-cell lung cancer is the leading cause of death among cancer patients. In this study, the delivery of doxorubicin by exosomes is tested by using a lung cancer cell line (H1299). Methods: Exosomes were isolated from H1299 cells using Invitrogen® exosome isolation protocol. Doxorubicin was loaded into exosomes by incubation and sonication methods of drug loading. Cancer cells were treated for a period of 72 hours with the free form of doxorubicin or doxorubicin-loaded within exosomes. The percent cell viability of cancer cells between treatments was compared by using MTT assay. Results: Percent cell viability results showed the anticancer effect of doxorubicin at various concentrations, either as free drugs or drugs carried by exosomes. However, free doxorubicin is very toxic to healthy cells. Compared to the untreated control group, both free doxorubicin and exosome-loaded doxorubicin caused substantial cancer cell death. The incubation method of loading doxorubicin into exosomes also showed slightly lower percent cell viability than the sonication method. Increasing concentration of doxorubicin (0.05 µg, 0.5 µg, 1 µg) resulted in a decrease in the viability of cancer cells, as low as 20% after 72 hours. Conclusion: This study reveals the potential for further investigation of exosomes as biocompatible drug delivery systems for various categories of drug molecules, such as small synthetic and biological molecules. References:
Exosomes as therapeutic drug carriers and delivery vehicles across biological membranes: current perspectives and future challenges. D. Ha, N. Yang, and V Nadithe. Acta Pharm Sin B. 2016 Jul; 6(4): 287–296.