Several patients develop chronic pain after surgical procedures, but the underlying mechanisms of the transition from acute to chronic states aren’t totally understood. Nowadays it’s well established that acute inflammation is accompanied by an active resolution program with specialized pro-resolving mediators. Manual therapy is a widely used therapeutic modality for pain management. In this study we investigated one of the underlying mechanisms of action.
Objective: evaluate the involvement of peripheral and spinal FPR2/ALX receptors on the anti-hyperalgesic effect of ankle joint mobilization (AJM) in a mouse model of postoperative pain.
Male Swiss mice (25-35g) were subjected to plantar incision (PI). Mechanical hyperalgesia was evaluated with the von Frey test; paw edema was assessed with a micrometer; and paw temperature through thermography. Animals were treated with AJM (either slow or rapid manipulation) or BML-111 (a FPR2/ALX receptor agonist) for 5 consecutive days. Other animal groups were injected (intraplantar route) with vehicle or WRW4 (a FPR2/ALX receptor antagonist) and treated with placebo AJM or AJM for 3 consecutive days.
Slow AJM reduced hyperalgesia in a mouse model of postoperative pain. Results are mediated, at least in part, by peripheral and spinal FPR2/ALX receptors.
Daniel Fernandes Martins– Professor, University of Southern Santa Catarina
Lisandro Ceci– Student, UNISUL
Afonso Salgado– Associate Researcher, University of Southern Santa Catarina
Daiana Cristina Salm– Professor, University of Southern Santa Catarina
Daniela Ludtke– PhD Student, UNISUL
Júlia Koerich– Student, UNISUL
Gustavo Mazzardo– Student, UNISUL
Kamilla Frech– Student, Unisul
Rodolfo Parreira– PhD Student, Instituto Salgado
Carlos Omura– Student, UNISUL
Francisco Cidral Filho– Associate Researcher, University of Southern Santa Catarina - Brazil
Leidiane Martins– Doutora, UFSC