Postdoctoral Fellow Institute of Plant and Microbial Biology/Institute of Molecular Biology
Rice repetitive proline-rich proteins (RePRPs) are known as Intrinsically disordered proteins (IDPs) that may harbor various regulatory functions due to the lack of fixed protein structures. Our previous studies indicated that RePRPs are induced by abiotic stresses and essential for proper root development. By integrating transcriptomic and proteomic analysis, we have discovered that genes respond to drought and anti-fungal defense were highly up-regulated under ectopic expression or RePRP2 (RePRP2OX). Phenotypic assessments indicated that RePRP2OX retained over 80% survival rate under extreme drought condition and outlived wild type (TNG67) and RePRP-RNAi transgenic rice. The underlying mechanism was likely due to more efficient water usage through increasing root aquaporin expression and lignin content. RePRP2OX lines had elevated levels of chitinases and phytoalexin biosynthesis genes and displayed resistance against oomycete pathogen Pythium arrhenomanes and rice blast fungus Magnaporthe oryzae. We propose that RePRPs serve as integrators of both abiotic and biotic stress responses and sustain plant growth under unfavorable conditions.
Coauthors: I-Chieh Tseng – Academia Sinica;Ching-Lan Wang – Academia Sinica;Tuan-Hua Ho – Academia Sinica