Description: What’s Growing in your Operating Room? Infection control, proper environmental cleaning, and turnover time are extremely important in the operating room (OR). At our institution we currently meet the turnover time criteria, however the sequential method of cleaning our OR’s was not being followed. As part of our Nurse Residency Program we developed an evidence based project that focused on the effectiveness of our current cleaning process in the OR. In collaboration with our Infection Prevention team, the project consisted of culture swabbing and black light testing of four high touch areas in the OR’s. Initial testing of high touch areas yielded results which showed growth on agar plates and black light evidence that the cleaning was subpar. It was clear from the results that critical surfaces were not being cleaned effectively. The staff consisting of nurses, surgical techs, and operating room assistants were educated on the proper sequential cleaning and process of effective cleaning of the OR’s during turnover. We emphasized high touch areas based on AORN recommendations. Follow up testing revealed a significant drop in growth on agar plates and decreased black light residue on high touch areas. This outcome resulted in a standardized cleaning process and the development of a resource to guide the staff to clean in a sequential and effective manner.