In emulsion gels, flow behaviour could be restricted due to repulsive jamming or attractive droplet aggregation. For nanoemulsions, repulsive jamming could be induced by decreasing droplet size and increasing interfacial shell-layer thickness. In this work positively charged chitosan with different degree of deacetylation (DDA 50 and 93%) was used to form bilayer electrostatic deposition on negatively charged Citrem (citric acid esters of monoglycerides)-stabilized oil droplets with the aim to determine the influence of interfacial shell-layer on repulsive gelation. The primary Citrem-stabilized liquid nanoemulsions (d32<200 nm) were prepared by high-pressure homogenization. After removal of excess Citrem by multiple centrifugations, secondary nanoemulsions were fabricated by adding different concentrations (0-0.25wt%) of chitosan at pH 4. With an increase in DDA93 chitosan concentration, negative charge (-48.0mV) of primary emulsion decreased to zero at 0.075wt%, increased to +51.9mV at 0.2wt% and remained unchanged thereafter. However, DDA50 chitosan showed a lower magnitude of positive charge and reached a maximum of +40.1mV at 0.2wt%. Accordingly, the microstructure changed from free-flowing Citrem-stabilized nanodroplets to aggregated droplets at ≤0.15wt%, and uniformly coated droplets at ≥0.2wt% chitosan. Emulsion viscoelasticity also went through a maximum which could be attributed to weak gels without chitosan, followed by strong aggregated gels between 0.05-0.15wt% chitosan, and smooth gels beyond 0.2wt% chitosan. With DDA50, viscoelastic maxima shifted towards a higher concentration of chitosan. Lipid digestibility of all bilayer nanoemulsions was decreased significantly compared to the primary nanoemulsion. Such gelation in bilayer nanoemulsions using a combined electrostatic and steric repulsion can serve as an attractive option to produce low-fat products.