Identification of major gangliosides species in human milk and infant formula
Thursday, July 2, 2020
12:10 PM – 12:35 PM CDT
Cynthia Marmet, Emmanuelle Bertschy, Claude Billeaud, Maria Jose Costeira, Magnus Domellöf, Mireille Vanpee, Jonathan O'Regan, Joanna E. Bettler
Nestlé Research Vers-chez-les-Blanc; Nestlé Research, Vers-chez-les-Blanc; University of Bordeaux ; Neonatology, Hospital Senhora da Oliveira Guimaraes; Dept. of Clinical Sciences, Umeå University; Astrid Lindgren Children's Hospital, Karolinska ; Nestlé Development Centre Askeaton, Limerick; Consultant for Global Scientific Affairs, Nestlé Nutrition, Vevey
Gangliosides (GD) are glycosphingolipids, which play an important role in brain and intestinal immune development. GD are synthetized endogenously and found in food such as meat and dairy products; in infancy, GD are provided by human milk. Breastfed babies were found to have a higher GD content in their frontal cortices than formula fed babies, thought to support neuronal function as for example myelination, synaptogenesis and memory formation. GD in breastmilk are also thought to influence the gut microbiota and interfere with pathogen binding in the intestine. The enrichment of infant formula with alpha-lactalbumin, provides a source of GD, due to a specific manufacturing process, with level similar to those of human milk, however, little is known about GD chemical similarity. In this study, we aimed at identifying the most abundant GD species in human milk and infant formula enriched with alpha-lactalbumin. High resolution mass spectrometry was used to elucidate the fragmentation pathway of GD allowing the identification of GD species. In both, human milk and infant formula, GD3 and GM3 were the most abundant GD classes and similar species were identified in the analyzed matrices.