Principal Research Officer Malaysian Palm Oil Board, Malaysia
Objective / Hypothesis :
Due to their minute concentrations, analyses of minor components in oil usually involve tedious sample treatment and preparations. The lack of authentic standards also contributes to the difficulty in analyses and calibrations, especially for tocotrienols and individual palm carotenes. With advancement in technologies, the tedious steps of sample pretreatment prior to analyses can be drastically reduced. This is applicable to the groups of minor components (carotenes, tocols, sterols, coenzyme Q) as well as individual compounds in each of these groups.
A single comprehensive SFC method for analyses of palm carotenes, tocols, sterols and squalene is developed. LOD, LOQ, linear range as well as the calibrations of these minor components are taken into consideration. Methods were also developed to calibrate the individual palm tocols (tocopherols, tocomonoenol and tocotrienols), as well as individual palm carotenes in the absence of authentic standards.
A single comprehensive SFC method for the analyses of minor components in oils has been developed. Total analyses time, starting from sample pre-treatment, preparation and analyses of different groups of components which often require different analytical tools / detection is drastically reduced. Individual palm tocols and carotenes were also analysed and calibrated in the absence of authentic standards. Crosscheck conducted revealed the methods to be accurate and reliable.
The method developed for the single analyses of palm minor components is rapid, reliable and accurate. This method is able to reduce the duration needed for analyses, thus saving time, cost and reducing backlog.