Molecular Insights from the Accelerating Medicines Partnership (AMP): Systemic Lupus Erythematosus

Moderator(s)

• 

  Deepak Rao, MD, PhD

  Brigham and Women's Hospital

Speaker(s)

• DH

  David Hildeman, PhD

  Cincinnati Children's Hospital
• AA

  Arnon Arazi, PhD

  Feinstein Institutes for Medical Research
• 

  Andrea Fava, MD

  Johns Hopkins University
• 

  Jill Buyon, MD

  NYU School of Medicine

In Phase 2 of the Accelerating Medicines Partnership study of systemic lupus erythematosus, over 150 patients with lupus nephritis were recruited for collection of clinical measures as well as kidney biopsies, blood, serum and urine. Kidney biopsies are being analyzed by both whole cell single-cell RNA-seq and nuclear RNA-seq. Cells from peripheral blood are being analyzed by mass cytometry and single cell RNA-seq. Both cells and fluid from urine are been assayed for urine biomarkers of kidney disease. This session will discuss the methods used to generate data and present findings from the ongoing analyses of kidney and urine.

Learning Objectives:

• Review the methods used to analyze kidney biopsy samples by single cell RNA-seq
• Discuss the immune and parenchymal cell features of lupus nephritis identified by single cell RNA-seq
• Describe the abnormalities in urine of lupus nephritis patients identified by urine proteomics
• Summarize the progress of the AMP SLE Network and its potential relevance in patient stratification and targeted therapy

Presentations:

• 10:00 AM – 10:10 AM ET

  Getting Single Cell RNA-Sequencing Data From Human Kidney Biopsies
  David Hildeman, PhD – Cincinnati Children's Hospital
• 10:10 AM – 10:20 AM ET

  Single Cell Transcriptomic Analyses of Lupus Nephritis Kidney
  Arnon Arazi, PhD – Feinstein Institutes for Medical Research
• 10:20 AM – 10:30 AM ET

  Urine Proteomics in Lupus Nephritis: Implications for a Liquid Biopsy
  Andrea Fava, MD – Johns Hopkins University
• 10:30 AM – 10:45 AM ET

  What Have We Learned About SLE from AMP, and Where Are We Going?
  Jill Buyon, MD – NYU School of Medicine
• 10:45 AM – 11:00 AM ET

  Q&A