Category: Professional Posters
Purpose: This study examines the effects of lactoferrin and black seed oil on microorganisms, as well as the extent to which they inhibit the growth of bacteria. Resistance to antibiotics has been outlined in previous research; therefore, a new strategy is required for sourcing natural biological materials for the extraction of antimicrobial peptides. The current research study addresses camel lactoferrin by evaluating its mechanisms of antimicrobial activity. This activity was observed in the contexts of Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumonia, Streptococcus pyogenes and Candida albicans.
Methods: Throughout study, we hypothesize that lactoferrin and NG exhibit a hydrolase- like antimicrobial action that functions principally as an isolate free from iron. Thus, it is necessary to remove some crucial substrates that are necessary for the evolution of bacteria within the bacterial membrane, as well as for binding to the lipopolysaccharide of bacterial walls. Consequently, the membrane will lose its rigidity, leading to the death of bacterial cells.
Disk diffusion method
A variety of systems can be used to target the antimicrobial frailty of a microorganism pathogen. The circle dissemination strategy represents a change to the Kirby-Bauer method.. If performed unequivocally, this system yields information that can anticipate in vivo adequacy of the referenced medication. However, despite circle dispersion’s ability to provide data allowing most antimicrobial specialists to translate a strain as defenseless, halfway or safe, this approach does not give exact data on negligible inhibitory fixation.
GOR IV prediction method
Specific secondary structures of mutant and native peptides were predicted through application of the GOR IV algorithm. The quality of the data supplied by the databases was verified.
Furthermore, the capacity of amino acids to generate given structures was also evaluated by the GOR IV calculated based on whether adjacent amino acids had previously formed the particular structures.
Results: This study has determined various results concerning the peptides and their effects on microorganisms in the context of positive bacteria and gram-negative bacteria, as well as for nigella sativa oil (BSO). The study has also found results for the mixture of the peptide and the Nigella sativa oil in the context of the microorganisms.
It has been found that lactoferrin is effective in bacterial applications. Gram-positive bacteria has also been found to be more efficient when the synergistic action of camel lactoferrin (SAoCL) mutant peptide is used with NS oil.
Conclusion: This study developed and maintained hypotheses related to the complexity of lactoferrin. It also showed that native and mutant aspects have direct effects on bacteria.
This study has achieved promising outcomes related to the analysis of the mutant peptide.
Using the disc diffusion method, crucial areas of inhibition against tested oral streptococci were identified in black seed oil. The present research has uncovered the effects of various pharmaceutical iterations of alkaloids on the antibacterial properties of Nigella sativa oil in relation to gram-positive bacteria. It was shown that these bacteria had greater sensitivity than gram-negative bacteria, with well-defined inhibition zones.