Micro- and Nanotechnologies
Droplet-based microfluidic has led to the development of highly powerful systems that represent a new paradigm in High-Throughput Screening where individual assays are compartmentalized within microdroplet microreactors. By combining a decrease of assay volume and an increase of throughput, this technology goes beyond the capabilities of conventional screening systems. Droplets can be produced as independent microreactors that can be further actuated and analyzed at very high throughput (several thousands to ten of thousands per seconds). Added to the flexibility and versatibility of platform designs, such progress in sub-nanoliter droplet manipulation allows for a level of control that was hitherto impossible.
We will show how droplet based microfluidics allows to perform single cell experiments with high efficiency and high throughput by presenting several recently developed droplet-based strategies. In particular, the capabilities of the newly developed microfluidic platforms for single cell encapsulation, phenotypic characterization and multiplex sorting. Finally, we will emphasize on the potential applications of such platforms for cancer research and resistance to treatment analysis.