The European Research infrastructure EU-OPENSCREEN was founded with support of ist member countries and the European Comission. Its distributed character offers complementary knowledge, expertise and instrumentation in the field of chemical biology from 20 European partner institutes while ist open working model ensures that academia and industry can readily access EU-OPENSCREEN´s collection of chemical compounds, equipment and associated screening data.
This work describes our collaborative effort between different medicinal and computational chemistry sites in Europe to set up a protocol for the rational design of a general purpose screening library against novel biological targets, consisting of about 100.000 commercial small molecules. The molecules were first pre-selected to ensure chemical stability, solubility and other screening-compliant physicochemical properties as well as absence of reactive compounds. Then the collaborating groups applied different methods to create diverse sub-libraries according to their own inhouse expertise, aimed at providing hits for a wide variety of drugable targets. Quality of approved vendors, cost and compound availability were other factors which shaped the content of the final screening library.