Preclinical Development – Chemical
2019 PharmSci 360
Layer-by-layer modification of a nanoparticle’s surface can be a simple process that involves mixing of a nanoparticle substrate with weak polyelectrolytes, followed by purification through dialysis, centrifugation, or diafiltration. The nanoparticle core can contain therapeutic payloads and/or imaging agents. The alternating charge layers of polyelectrolytes can also contain therapeutic payloads, and can add additional functionality through modular design. Biocompatibility can be improved, and specific receptor targeting can be achieved by introduction of certain polymers as the terminal layers of the nanoparticle. In this talk, the application of layer-by-layer surface modification of polystyrene nanoparticles in the treatment of colorectal cancer, both alone and in combination with radiation therapy, will be discussed. This presentation includes details of the nanoparticle formulation, in vitro assessment of cell uptake and viability, and tumor inhibition in a murine model.