Bioanalytics – Biomolecular
2019 PharmSci 360
There are several different platforms for quantification of protein biomarkers in the sub-picogram range, and one of the platforms is single-molecule array (Simoa) digital ELISA. We had used this platform to measure mouse cytokine levels in our preclinical immunology and immuno-Oncology programs since the levels of some cytokines were too low to be measured by other platforms such as Luminex and MSD platforms. Simoa was also used to measure changes of PD biomarkers over time course after administration of biologics in the same mice since it required just several microliter sera from mouse microsampling in each time point; therefore, it reduced variability and the number of mice used in the study. We also developed free (drug-unbound) and total (drug-bound and unbound) pharmacodynamics biomarker assays in Simoa platform to asses target engagement in plasma.