Bioanalytics – Biomolecular
2019 PharmSci 360
Interleukin-25 a member of the IL-17 cytokine family, is a proinflammatory cytokine favoring Th2-type immune response and stimulates the production of various other cytokines (incl. IL-8, IL-4, IL-5, IL-13) in multiple tissues. Therefore IL-25 is a central molecule controlling immunity and has been associated with chronic inflammation and allergic diseases such as asthma or inflammatory bowel disease, and parasitic infection. Measuring endogenous IL-25 in biological specimen is challenging due to matrix interaction of the biomarker. In ligand-binding assay (LBA) development, the challenge is to identify the adequate antibody tools and to provide the needed assay sensitivity to measure biomarker levels for all sample cohorts under study, especially at low concentrations - a limitation on most LBA platforms. Here we present selection of antibodies, development of an IL-25 biomarker LBA assay, and fit-for-purpose validation providing the best signal-to-noise ratio and needed sensitivity in serum samples of autoimmune patients and healthy volunteers.