Preclinical Development – Chemical
2019 PharmSci 360
We introduce a method to measure multiple characteristics of particle-based therapeutics, including:
1. The suspension concentration of nanoparticles.
2. The size distribution of nanoparticles.
3. The encapsulation of drug inside the liposomal and the dose of drug.
The technical aspects of our work including:
1. Device-enhanced optical microscopy. Fabricate a microfluidic device by microelectromechanical systems to well control the device dimension for optical microscopy.
2. Single-particle tracking and sizing. Apply modified Stokes-Einstein equation to perform the nanoparticle tracking analysis about the information of sizing.
3. Light-scattering and fluorescence. Utilize transluminescence darkfield microscopy to monitor the nanoparticle rely on its strong light-scattering, and epifluorescence microscopy to recognize the drugs inside the liposomal simultaneously. The fluorescence signal is used to determine how many drugs inside.
4. Precision, accuracy, and efficiency of this methodology. Monitor the long trajectories of a nanoparticle and the many nanoparticles to increase the precision. Fabricated microfluidic devices provide the good depth control and support position references for localization to improve the measurement accuracy. The reusable devices and the facilitate optical microscope give the great efficiency of nanoparticle measurement.