Bioanalytics – Chemical
2019 PharmSci 360
A quantitative method has been developed and validated to handle bioanalysis of a triphophate active drug metabolite from human intestinal tissue. Unique sample handling and processing measures were implemented to enable reproducible recovery and quantitation accuracy of the triphosphorylated analyte from the matrix of interest within determined stability limitations. Sample state, storage conditions, container systems, homogenization bead selection, and sample processing protocols were critical to mitigate non-specific binding of the phosphorylated analyte and to assay samples within known stability limitations. This method was used to support the analysis of biopsy tissues from human clinical trials to treat human noroviruses.