Category: Preclinical Development
Purpose: Exosomes transfer genetic and epigenetic cargos including microRNAs (miRs) to recipient cells. While miR profiling approach has been used to segregate prostate cancer (PCa) patients’ clinical outcomes, yet differential exosomes-associated cargo that can stratify these patients based on their races and aggressiveness are still unmet need. Our aim here is to evaluate the role of exosomes-associated miRs in PCa cell lines and serum samples collected from PCa patients and healthy subjects.
Methods: miRs profiling was performed for exosomes derived from serum samples collected from African and Caucasian American PCa patients at different tumor grades. Total RNA was extracted from exosomes and Real-Time PCR was performed to validate top miR candidates. The sensitivity and specificity of these miRs were assessed.
Results: Not only the expression levels of miR-7704, miR-6068, and miR-6076 were significantly higher (P˂0.05) in a large panel of PCa cells compared to RWPE1 cells but also in PCa patients (n=60) compared to healthy control subjects (n=30). Interestingly, miR-7704 recorded the highest diagnostic performance between high and low Gleason score (AUC=0.87 and 87% sensitivity & 80% specificity). The diagnostic accuracy was increased when the three miRs were combined (AUC=0.94 and 92% sensitivity & 89% specificity).
Conclusion: Differentially expressed exosome-associated miRs serve as a biomarker, which can stratify PCa patients from healthy subjects (miR-7704, -6068, and -6076) and high from low Gleason score (miR-7704). Combination of these miRs improves the diagnostic accuracy of these biomarkers.
Mohamed Gaballah– Kingsville, Texas
Shahenda Mahgoub– cairo, Al Qahirah, Egypt
Tamer Fandy– Associate Professor, University of charleston, school of Pharmacy, Charleston, West Virginia
Hamdy Ali– kingsville, Texas
Hamed Ali– Kingsville, Texas
Zeinab Hassan– cairo, Al Qahirah, Egypt
Zakaria Abd Elmageed– kingsville, Texas
Rofaida Gaballa– Kingsville, Texas