Bioanalytics – Biomolecular
2019 PharmSci 360
Pulmonary arterial hypertension (PAH) is a fatal disease; early diagnosis is vital to reduce mortality. Circulating Endothelial Cells (CECs) were proposed as a biomarker for diagnosis. However, current tools for the isolation of CECs are complex. Here we described the design and clinical utilization of a biodegradable nano-film coated microfluidic chip for CECs detection and characterization of isolated cells.
High cell-capturing efficiencies, 71% purity, and 94.2% release efficiency are the characteristics of our device. In PAH-induced rats, over a 12-week period, the number CECs raised as the disease worsened. The clinical application showed the numbers of CECs in PAH patients were five times greater than that in controls, Doppel antibody only identifies PAH-ECs, and Captured CECs of PAH patients originated from diseased pulmonary arteries. There is a strong correlation between the chip and flow cytometric methods.
This microfluidic device represents a potential tool for early diagnosis and monitoring of PAH.