Bioanalytics – Chemical

2019 PharmSci 360

Optimized Microflow LC-MS/MS Methods for the Bioanalysis of Large Molecules

Speaker(s)

• 

  Shane Needham, PhD

  Alturas Analytics, Inc.
  Moscow, Idaho

Here we present optimized approaches for the LC-MS/MS bioanalysis of ADC’s, peptides and proteins. We will discuss the use of microflow LC-MS. We will discuss the importance of using ESI sources designed for microflow-LC-MS. We will also present how micro solid-phase extraction and immunocapture can be used for optimal analysis of peptides and proteins by LC-MS. Approaches for method development, validation and sample analysis will be described.

Learning Objectives:

• The participants will better understand optimal approaches for the bioanalysis of large molecules by LC-MS/MS.
• The participants will be able to discuss method development approaches for the LC-MS bioanalysis of large molecules
• The participants will be able to explore the techniques we discuss and determine their fit in the work-flow of their laboratory