Comparison of C57Bl/6, NSG-PBMC, CD34-NSG, and SGM3 mice models for immune checkpoints.
Wei Zeng, Xiaohua Wu, Hana Baker PhD, and Yang Zhao PhD*
*Correspondence author and principle investigator: firstname.lastname@example.org
Eli Lilly and Co
Lilly Corporate Center, Indianapolis, IN 46225
Immune checkpoint receptors have become an important class of therapeutic targets. Intensive research has found major differences between mice immune system and human immune system. In order to identify suitable models for particular research purpose, this study compared C57Bl/6 with complete mice immune system, NSG-PBMC that has mostly human T cells engrafted, CD34-NSG that has most human immune cell compartments and SGM3 that introduced human cytokines as transgenes. A model based on original findings from Tasuku Honjo and co demonstrated anti-viral efficacy of commercial immune checkpoint receptor antagonist in C57Bl/6. Similar responses are observed in NSG-PBMC.
We also looked at various immune cell compartments of CD34-NSG and SGM3 by flow cytometry analysis. Major differences between the two models have been identified including the abundance of Treg and pDC. Expression of several checkpoint receptors have been examined in different immune cell compartments of these models. These findings provide useful insights that will expedite immune checkpoint research in both Onco-Immunology and Autoimmunity.