Bone marrow or stem cell transplantation
Background: The immunomodulatory potential of mesenchymal stem cells (MSCs) have been largely discussed while the specificity with the sources have not been enumerated.
Methods: MSCs were isolated from umbilical cord blood (UCB), dental pulp (DP) and liposuction material (LS). Their cytokine profile was enumerated and compared after co-culture with activated neutrophils. Cytokine profiling included IL-1a, IL-2, IL-4, IL-6, IL-8, TNF-a, IFN-g, and TGF-b and added we assessed cellular proliferation, cell death and differentiation to selection pressure.
Results: The results showed a comparable cytokine response among all types in response to activated neutrophil co-culture, while specifically the LSMSCs cytokine response with TNF-a and IFN-g was high owing to its mature cellular phenotype compared with UCBMSCs and DPMSCs. We did not observe any significant change or alterations in cellular viability or proliferation between LP/DP/UCB MSCs when co-cultured with activated neutrophils. Further when the MSCs were directly activated with lipopolysaccharide (LPS), which served as control, however did not induce a rapid proliferation, while cell death was evidenced in all the three types of MSCs. We then induced all the types of neutrophil exposed MSCs for osteogenic, adipogenic and chondrogenic differentiation and observed that, all the MSCs devoid of the sources differentiated, however a significant rapid turnover of DPMSCs positive for osteogenic markers and LSMSCs to adipogenic markers were observed over UCB MSCs.
Conclusion: Taken together these results suggest that though all the MSCs devoid of sources are activated DPMSCs and LSMSCs are niche orientated while UCBMSCs responds equivocally due to its comparative naïve nature.