Immunity & infection
Rotavirus remains the principal cause of severe diarrhoea among infants worldwide. Study aimed at determining the prevalence of rotavirus infection and blood levels of immunoglobulins among children with acute gastroenteritis in Nnewi. The subjects comprised of 43 symptomatic rotavirus positive, 33 symptomatic rotavirus negative, 14 asymptomatic rotavirus positive, 31 asymptomatic rotavirus negative. They were 1 – 59 months of age. Blood samples were collected Rotavirus screening was determined by enzyme-linked immunosorbent assay, immunoglobulin levels by turbidimetric technique. The prevalence rate of rotavirus was 47.16%. Rotavirus gastroenteritis cases occurred highest (40.4%) in children aged 10-18 months. Seropositivity of rotavirus recorded highest among children whose parents had tertiary school education (28.4%). But level of education had no significant impact on rotavirus status χ2 (1, N = 121) = 3.18, p = 0.08. Rotavirus seropositivity compared between the exclusive and non exclusive breast fed babies, showed no significant difference (P=0.880). Mean IgM mg/dl levels compared among, groups 1 (323.52±76.89), 2 (308.48±79.618), 3 (318.83±67.41) and 4 (299.31±64.58) showed no significant difference (f value=0.718, p=0.543). Immunoglobulin G mg/dl mean levels in group1 (1755.16±229.55), group 2 (1751.12±236.07), group 3 (1770.66±216.02) and group 4 (1730.428±234.93) were not significant (f =0.118, p=0.949). Immunoglobulin A mg/dl mean levels; groups 1 (110.175±64.09), 2 (103.09±58.27), 3 (132.965±55.06) and 4 (105.437±60.31) were not significantly different (f =0.871, p=0.459). In conclusion, the prevalence of rotavirus was 47.16 in Nnewi. There was no significant deference in immunoglobulin levels among the groups.