Title: Leveraging Flow Cytometry-based Functional Assays in Translational Research
Speaker: Jean-Marc Busnel, Ph.D., Senior Staff Research Scientist
Beckman Coulter Life Sciences
Flow cytometry-based functional assays represent unique tools in the bioanalytical landscape. Flexible and powerful, they can be adapted to characterize in detail the response of the immune system to virtually any kind of stimulus if it is not toxic to the sample considered. While they are most often carried out on simplified systems such as cell lines and/or purified primary cells, flow cytometry-based functional assays can also be performed on whole blood, which certainly represents the most powerful ex vivo model as it not only involves immune cells but also soluble components such as antibodies, cytokines, metabolites, etc.
Considering several examples, we would like to share our efforts in streamlining and democratizing such approaches. The emphasis will mainly be placed on two applications - allergy research and biologics characterization.
It will first be shown that basophil activation testing (BAT) workflows can be drastically simplified without sacrificing on performances and that it can further be miniaturized and automated on a 96 well-plate format. On the biologics side, we will discuss some of our recent work around the development of new ex vivo characterization tools serving different purposes such as mAbs comparability assessment, stability studies and/or mechanisms of action (MOA) characterization.