TITLE: Simultaneous analysis of proteins and gene expression in single cells - novel applications of TotalSeq™ oligo-antibody conjugates and Chromium Single Cell Solutions with Feature Barcoding technology
SPEAKERS: Chris Gould, PhD, BioLegend & Kasandra Burgos, PhD, 10X Genomics
ABSTRACT: Join 10x Genomics and BioLegend for an introduction to how the simultaneous analysis of single cell gene expression, cellular characterization, and immune profiling are revolutionizing biological research. Learn how TotalSeq™ antibody-oligonucleotide conjugates, along with the Chromium Single Cell Gene Expression Solution and Single Cell Immune Profiling Solution, enable the generation of both single cell transcriptomic and proteomic data simultaneously with unprecedented capacity for multiplexed protein analysis at a single cell level. Here we illustrate how these reagents can be used together to identify new biomarkers in immune-oncology research, and the utility of highly multiplexed assays up to 220 protein markers. In addition, we show the use of cell hashing reagents, which enable sample multiplexing, resulting in reduced variability, and optimization of complex experimental designs. With the development of the TotalSeq™ reagent suite and its integration with Chromium Solutions, we hope to facilitate the next wave of legendary discovery in biomedical research.