Background: Bronchiolitis obliterans syndrome is the main limitation for long-term survival after lung transplantation. Recent data suggest that some specific B cell populations are associated with long-term graft acceptance and may serve as biomarkers in other organ transplantation. We aimed to monitor B cell profile during early development of bronchiolitis obliterans syndrome after lung transplantation.
Methods: B cell longitudinal profile was analyzed in peripheral blood mononuclear cells from patients with bronchiolitis obliterans syndrome and patients who remained stable regarding pathology development after lung transplantation.
Results: CD24hiCD38hi transitional B cells were only increased in stable patients to reach a peak 24 months after transplantation, whereas remaining unchanged in patients who developed a bronchiolitis obliterans syndrome. We showed that these CD24hiCD38hi transitional B cells specifically secrete IL-10 and express the CD9marker. Thus, patients with a total CD9+ B cell frequency below 6.6% displayed significantly higher incidence of bronchiolitis obliterans syndrome.
Conclusions: These data are the first to associate IL-10-secreting CD24hiCD38hi transitional B cells expressing CD9+ with better allograft outcome in lung transplanted patients. The identification of CD9-expressing B cells as a contributor to a favorable environment essential for the maintenance of long-term stable graft function and as a new predictive biomarker of bronchiolitis obliterans syndrome–free survival provide new strategies for treatment of the pathology and its prediction.
Carole Brosseau– Centre de Recherche en Transplantation et Immunologie
Richard Danger– post-doctorate, CRTI UMR1064, INSERM, Université de Nantes
Maxim Durand– Former Phd student, Center for Research in Transplantation and Immunology - INSERM UMR1064 - Nantes
Eugénie Durand– Center for Research in Transplantation and Immunology - INSERM UMR1064 - Nantes
Aurore Foureau– CHU Nantes
Antoine Magnan– CHU Nantes
Sophie Brouard– Centre de Recherche en Transplantation et Immunologie (CRTI) UMR 1064, INSERM, Université de Nantes, ITUN, CHU de Nantes