Presentation Authors: Adriana Vidal*, Los Angeles, CA, Taofik Oyenkunle, Lauren Howard, Amanda DeHoedt, Durham, NC, Emanuela Taioli, New York, NY, Jay Fowke, Memphis, TN, Charles Drake, New York, NY, Stephen Freedland, Los Angeles, CA
Introduction: We hypothesized the cytokine profile of black and white men undergoing a prostate biopsy would be different and that systemic inflammation would be associated with higher prostate cancer (PC) risk.
Methods: We prospectively recruited incident prostate biopsy positive (n=188) and biopsy negative men (n=185) at the Durham Veteran Affairs, from 2007-2018. We used the V-PLEX Human Cytokine 36-Plex Kit from MesoScale to measure 36 cytokines and chemokines in the serum of those men collected prior to biopsy. We measured serum cytokines/chemokines expression by race. Using logistic regression, we examined the association between cytokines/chemokines and overall PC risk and stratified analyses by race.
Results: Cases had higher PSA (7.2 vs. 5.5 ng/ml, p < 0.001), although there were no differences in BMI, age, and race between biopsy positive and biopsy negative men (all pâ‰¥0.69). Among all men the pro-inflammatory cytokines IL-10, IL-13, IL-1Î² and TNF-Î±, were differently expressed in black versus white men, all p-interaction â‰¤0.016. Other cytokines and chemokines differently expressed by race were: GM-CSF, IL-12/IL23p40, IL-15, TNF-Î², Eotaxin-3, MDC, IL-21, and IL-31, all p-interaction â‰¤0.027. IL-13 was associated with higher risk of PC in black men (OR=1.57, 95%CI 1.08-2.28, p=0.001) and lower PC risk in white men (OR=0.71, 95%CI 0.54-0.93, p=0.012). IL-10, IL-1Î², Eotaxin-3, IL-21, and IL-31 were associated with higher PC risk (all pâ‰¤0.049) in black men but no associations were found in white men. TNF-Î±, GM-CSF, IL-12/IL-23p40, IL-15, TNF-Î², and MDC, were associated with increased risk of PC (all pâ‰¤0.036) in white but not in black men.
Conclusions: In agreement with our hypothesis, pro-inflammatory cytokines measured prior to a prostate biopsy were differently expressed in black versus white men. Several cytokines were differently associated with PC risk by race. Regardless of race, interventions aimed at reducing systemic inflammation, e.g. through diet, may help reduce the risk of PC. Future studies are needed to confirm these results.
Source of Funding: Supported by National Institutes of Health; Grant number: K24 CA160653, and Department of Defense Award: DOD W81XWH-16-1-0750. Adriana Vidal was supported by a Research Scholar Grant, RSG-18-018-01 - CPHPS, from the American Cancer Society.