Presentation Authors: Tamara Jamaspishvili*, Palak Patel, KINGSTON, Canada, Yi Niu, Dalian , China, People's Republic of, Thiago Vidotto, Isabelle Caven, Rachel Livergant, Winnie Fu, Kingston, Canada, Veronique Ouellet, Montreal , Canada, Clarissa Picanço, São Paulo, Brazil, Madhuri Koti, Nathan How, Kingston, Canada, Fred Saad, Anne-Marie Mes-Masson, Montreal , Canada, Tamara Lotan, Baltimore , MD, Jeremy Squire, São Paulo, Brazil, Yingwei Peng, David Berman, Kingston, Canada, Rodolfo Reis, São Paulo, Brazil
Introduction: Loss of the PTEN tumor suppressor is a powerful prognostic biomarker in prostate cancer. However, the significance of tumour heterogeneity and partial loss are not clearly defined, nor are interactions between PTEN loss and the TMPRSS2-ERG fusions, the most common genetic aberration found in prostate cancer. Taking into account TMPRSS2-ERG status, we aimed to define and quantify patterns of PTEN loss that best account for the risk of recurrence in low and intermediate-risk prostate cancer patients who underwent radical prostatectomy.
Methods: Tissue microarrays comprising a training (n=410) and validation (n=272) cohorts were constructed and PTEN protein levels were measured using automated clinical grade immunohistochemistry assays. PTEN loss was quantified per cancer cell and per cancer core using digital and visual scoring. Thresholds for PTEN loss were determined by log-rank statistics and Kaplan-Meier survival estimator. Cox's proportional hazards models were used to determine the prognostic significance of selected cut-offs of PTEN loss along with multiple pathological and clinical variables.
Results: PTEN loss in >65% of cancer cells (digital scoring)/per case or >50% of TMA cancer cores/per case (visual scoring) were associated with a 50% reduction in recurrence free survival (RFS) (% cells, HR=4.22, p < 0.001 and % cores, HR=2.75, p=0.002 in multivariate analysis, respectively). Median RFS was 10.2 yrs for any PTEN loss (p < 0.001) vs 5.4 yrs for >65% cells with PTEN loss (p < 0.001) in Kaplan-Meier analysis. Cases with PTEN loss but without TMPRSS2-ERG fusion had the shortest RFS (4.1 yrs) compared to cases with TMPRSS2-ERG fusion (10 yrs; p=0.001). Finally, PTEN loss was found almost exclusively in dominant tumor foci (50/54 cases).
Conclusions: Degree of PTEN protein loss is strongly associated with disease progression. PTEN loss is independently associated with increased risk of disease progression regardless ERG status. Its high level of intra-focal heterogeneity and strong association with dominant foci indicates that PTEN assessment is vulnerable to sampling error and might influence on prognostic assessment of biopsy samples. Any PTEN loss may not be a â€œred flagâ€ for poor prognosis. Quantitative assessment of PTEN loss may improve risk stratification of patients with localized prostate cancer.
Source of Funding: Work by T.J., P.P. and D.M.B. was awarded by Prostate Cancer Canada (PCC) and is proudly funded by the Movember Foundation-Grant #T2014-01. T.J. was supported by a Transformative Pathology Fellowship funded by the Ontario Institute for Cancer Research (OI