Presentation Authors: Meng Li, ZhongCheng Xin*, Beijing, China, People's Republic of
Introduction: CN injury includes many forms such as crush, transection, resection, cautery and freeze injury, which lead to different degrees of damage. In this study, we compared the pathological changes caused by bilateral cavernous nerve crush (BCNC) and bilateral cavernous nerve resection (BCNR)and to explore the therapeutic effect and mechanism of sildenafil.
Methods: In one experiment, 45 SD male rats were randomly assigned to three groups and underwent BCNC, BCNR or sham surgery. 5 rats in each group were tested for erectile function at the 1, 3, 5 week. In a second experiment, 50 rats were randomly assigned to four groups. 10 rats underwent sham surgery and received PEG400 (Sham group). 20 rats underwent BCNC and treated with PEG400 (BCNC group, n=10) or sildenafil (BCNC+sildenafil group, n=10), 20 rats underwent BCNR and treated with PEG400 (BCNR group, n=10) or sildenafil (BCNR+sildenafil group, n=10). Three weeks after surgery, Intracavernous pressure to mean arterial pressure (ICP/MAP) was measured and the middle cross-sections of penile was processed for immunohistochemistry, immunofluorescence, TUNEL, Massonâ€™s trichrome staining western blotting.
Results: ICP/MAP was significantly decreased in BCNC and BCNR group at 3 week. The smooth muscles in the BCNR group became hypertrophy and the expression of ê¤-SMA decreased significantly, as well as the nNOS and RECA-1. After sildenafil treated, ICP/MAP and the expression of ê¤-SMA, nNOS and RECA-1 were increased in both BCNC+Sildenafil and BCNR+Sildenafil group, but there was no significance in BCNR+Sildenafil group. Smooth muscle to collagen by Massonâ€™s trichrome staining showed no obvious difference. The apoptosis followed BCNR increased significantly and there was no obvious improvement after three weeksâ€™ treatment.
Conclusions: Nerve-sparing in prostatectomy is important and low dose and long-term use of sildenafil can accelerate the recovery of erectile function after BCNC.
Source of Funding: NFSC8167060244