Presentation Authors: Dae Keun Kim*, A Ra Jung, Jung Ki Jo, Seoul, Korea, Republic of, Hyung Ho Lee, Goyang, Korea, Republic of, Joon Chae Na, Woong Kyu Han, Sung Yul Park, Hong Sang Moon, Young Eun Yoon, Seoul, Korea, Republic of
Introduction: CORM-3 has organ protective effect in ischemic or inflammatory conditions. Using a single-kidney rat model, we evaluated renoprotective function of injection of CORM-3 in renal ischemia reperfusion injury.
Methods: The rats were randomly divided into three groups; sham (right nephrectomy), control (right nephrectomy and left renal ischemia), and CORM-3 (right nephrectomy and CORM-3 injection before left renal ischemia). For renal ischemia and reperfusion injury, just after right nephrectomy, we clamped left renal vessels for 30 to 90 minutes. In CORM-3 group, CORM-3 10mg was injected via caudal artery, followed by left renal artery clamping 1 hour later. After sacrifice of animals, kidney tissues and blood samples were evaluated to determine the renoprotective effect of CORM-3.
Results: Serum creatinine at one day after ischemia reperfusion showed ischemia time dependent increasements. In 30 minutes and 45 minutes experiments, there was no significant difference between the groups because of not enough renal damage, but in IRI injuries more than 60 minutes, CORM-3 group showed lower serum creatinine than control group. In the pathological review of the kidney tissues, the normal tubules and glomerulus were more conserved in the CORM-3 group. TUNEL staining showed fewer apoptotic renal tubular cells in the CORM-3 group compared with control.
Conclusions: Pretreatment with CORM-3 injection before renal ischemia reperfusion injury showed renoprotective effect. This could be a new strategy against renal ischemia reperfusion injury in partial nephrectomy or kidney transplantation.