Presentation Authors: Muqiu Zhang*, Zhenjie Zhu, Jindong Sheng, Jin Song, Peng Xiang, Hualin Song, Shuai Hu, Jie Jin, Beijing, China, People's Republic of
Introduction: Benign prostatic hyperplasiaï¼ˆBPHï¼‰ is a disease characterized by stroma hyperplasia. Our previous studies, stromaâ€“epithelium ratios and collagen deposition were prevalent in rapidly-progressed BPH patients (age â‰¤50 years old). Meanwhile, we also detected that senescent prostatic epithelial cells were associated with the number of fibriblast. The present study mainly investigates if senescent prostatic epithelial cells could influence the progression of BPH through inducing the growth of fibroblast.
Methods: Formalin-fixed paraffin-embedded (FFPE) prostate tissues obtained from three groups of patients (7 cases in each) were included in this study: (1) prostate tissues of patients with rapidly-progressed BPH, aged â‰¤50 years, obtained during the transurethral resection of the prostate (TURP); (2) age-matched prostate tissues, acquired from patients with bladder cancer, aged â‰¤50 years, who underwent radical cystectomy and prostatectomy and (3) prostate tissues of elderly patients with BPH, aged â‰¥70 years, obtained during TURP. Senescent epithelial cells were labeled by p16INK4a (senescent related gene) through immunohistochemistry. In vitro, we used doxorubicin to modeling epithelial cells (BPH-1 cells) aging. After 2 daysâ€™ culture of BPH-1 treated with doxorubicin, the conditioned media was harvested and used to evaluate the proliferation of primary prostatic stromal cells by Cell Counting Kit. Quantitative PCR was used to identify cytokines secreted by the BPH-1 cells treated with doxorubicin.
Results: p16INK4a stains almost in prostatic epithelial cells, and it was expressed stronger in rapidly-progressed BPH than in the other two groups. The conditioned media could accelerate the proliferation of primary prostatic stromal cells. Cytokines CCL-5ã€VEGFã€IL-10ã€IL-1aã€IL-4ã€IL-6ã€IL-8 were increased in senescent BPH-1 after being treated by doxorubicin. Senescent prostatic epithelial cells could influence the rapid progression of BPH by these cytokines.
Conclusions: Senescent prostatic epithelial cells may play a role in the progression of BPH through facilitating fibroblast proliferation.