Presentation Authors: Konstantin Polyakovskiy*, Natalia Potoldykova, Alexander Glukhov, Sergei Gordeev, Andrei Vinarov, Dmitrii Enikeev, Leonid Rapoport, Petr Glybochko, Moscow, Russian Federation
Introduction: The purpose of the study was to assess the feasibility of measuring telomerase activity in urinal sediment cells for non-invasive diagnosis of urinary bladder cancer (BC) and its relapses in the postoperative period.
Methods: The study enrolled 50 patients with BC. The control group was composed of 40 patients. Thirty-eight patients of the study group were subjected to transurethral resection (TUR), 5 patients underwent open bladder resection and 7 patients endured radical cystectomy. Histological examination revealed non-invasive bladder cancer in 32 patients and invasion into the muscular layer in 18 of 50 patients. The control group consisted of patients with renal cysts, chronic cystitis and benign prostate hyperplasia.To examine telomerase activity (TA), a total of 90 urine samples were taken from both the Study and Control group patients. TA in urine cells was determined by means of telomeric repeat amplification protocol (TRAP), real-time PCR and electrophoresis.Forty-three patients with preserved urinary bladder were postoperatively followed up for one year with the examination of TA and control cystoscopies each 3 months.
Results: In the Study group, TA was detected in 46 (92%) of the 50 urine specimens. In the control group, TA was revealed in 1 (0.4%) of the 40 patients. The obtained findings thus strongly suggest statistically significant prevalence of urinary bladder cancer patients with the presence of TA in urine (p=0.001).We also revealed a correlation between TA and tumour differentiation. In 16 urine samples in patients with G1, the average TA amounted to 0.7%, in 20 urine samples in G2, the mean TA amounted to 0.98%, and in 10 urine samples in G3, the mean TA amounted to 1.33%.Â Â In stage G1 y = -0.003463 + 0.4700 x strong significant correlation (p=0.0003). In stage G2 y = -0.01960 + 0.5456 x strong significant correlation (p=0.0001). In stage G3 y = 0.01370 + 0.5395 x strong significant correlation (p=0.02). The sensitivity and specificity of the method amounted to 92% and 97.5%, respectively.During the postoperativeÂ follow up of 43 patients with the preserved urinary bladder we revealed BC relapse in 17 patients (39,5%). TA in urine was present in 14 of 17 (82,35%) relapse cases and was negative in 3 of 17 cases (17,65%).
Conclusions: The preliminary data suggest the possibility of using telomerase activity in diagnosis of urinary bladder cancer and its relapses.
Source of Funding: Sechenov University