Presentation Authors: Maya Epifanova*, Badri Gvasalia, Maksim Durashov, Sergey Artemenko, Moscow, Russian Federation
Introduction: The purpose of the trial was to evaluate Platelet-rich plasma (PRP) modifications safety and effectiveness for treating experimentally induced azoospermia in rat model. Busulfan-induced azoospermia (BIA) model and Cisplatin-induced azoospermia (CIA) model were tested. CIA model failed because of its profound cytotoxic effect, distant rats death and irreversible thrombocytopenia. BIA model was used.
Methods: 80 adult male Wistar rats weighing 250-300g were randomly assigned in 5 equal groups. Rats of 4 experimental groups (EG) received 10 mg/kg intraperitoneal Busulfan injections twice to induce azoospermia. 0,9% saline solution was administered in peritoneal cavity of the control group (CG) rats. Each EG received one of the following PRP modification injection (50 ÂµL/testis) - Poor PRP, PRP, PRP activated with 10% of CaCl2 (a-PRP), Leukocyte- and Platelet-Rich Plasma (L-PRP). PRP was administered 1 time a week during 6 weeks following Busulfan injection. Whole blood for PRP processing was obtained from inbred donors. Rats reproductive organs weight, body weight, complete blood count (CBC), epididymal sperm count, sperm motility and morphology were assessed on 14th, 28th, 42nd day after PRP administration.
Results: Total body weight did not change. However, weight gain in EG was suppressed by 42nd day by comparison with CG. CBC in experimental groups was reduced by the second week after Busulfan injection and reached CG levels by 42nd day (p < 0.05). Reproductive organs mass was reduced in the EG (p < 0.05). Epididymal sperm count was increased in a-PRP, L-PRP groups compared to CG at 42nd day after receiving a-PRP and L-PRP (p < 0.05). Spermatozoa with abnormal morphology counts were increased by 42nd day (p < 0.05) and correlated with the decrease of their motility (p < 0.05) in EG. Histologically, there were severe seminiferous epithelium disorganization and testicular parenchyma swelling, as well as reduction of diameter of the seminiferous tubules (p < 0.05).
Conclusions: PRP-therapy is a safe method for rats&[prime] spermatogenesis recovery. The next aim for the trial is to evaluate PRP-therapy effectiveness for spermatogenesis recovery. Excessive spermatogenesis suppression was found in BIA rats. Reproductive cells damage and inflammation induction was due to frequently performed intratesticular PRP injection. The final assessment should be made on 60th day because of the amount of time taken to produce spermatozoa in the rat testis.