Presentation Authors: Shaheen Alanee*, Detroit, MI, Bradley Holland, Kristin Delfino, Danuta Dynda, Ahmed El-Zawahry, Andrea Braundmeier, Springfield, IL, kevin McVary, kevin McVary, Maywood, IL
Introduction: There is accumulating evidence that variations in the human microbiota may promote different benign disease conditions. Our goal was to examine the association between urinary and fecal microbial profiles and the different aspect of lower urinary tract symptoms (LUTS) in adult males.
Methods: We extracted total DNA from urine and fecal samples and collected international prostate symptom score (I-PSS) data from a group of adult males through an intuitional review board approved protocol. We then amplified the extracted DNA and sequenced it using bacterial 16S rRNA gene high-throughput next-generation sequencing platform, and analyzed microbial profiles for taxonomy to examine the association between the different operational taxonomy units (OTUs) and LUTS represented by the overall all I-PSS score and its subcomponents of storage, nocturia, voiding, and bother.
Results: We included 30 patients in our analysis (60 samples, one urine and one fecal per patient). Characteristics of the study subject&[prime]s LUTS are illustrated in table 1. Forty-eight fecal OTUs showed significant association with one or more of the I-PSS components; 27 with nocturia, 19 with bother, 16 with storage symptoms, and 9 with voiding complaints. The strongest association was between Lachnospiraceae Blautia, a bacteria that increases the availability of gut anxiolytic and antidepressant short chain fatty acids, and bother (correlation coefficient - 0.702). Ten urinary OTUs showed significant association with LUTS; 8 with nocturia, 1 with bother, 3 with storage, and 1 with void. The abundance of one urinary bacteria that belongs to a family previously shown to be enriched in patients with bladder cancer, Ruminococcaceae Gemmiger, was common to increased voiding, storage, and nocturia scores
Conclusions: Our prospective work finds plausible correlation between urinary and fecal microbiota and LUTS. However, due to the limited number of participants additional studies are needed to determine if the associations found in this research are applicable to the general population of patients affected by LUTS.
Source of Funding: Southern Illinois University School of Medicine, Research fund.